The allergen is bound to a solid support. The serum is prepared from a blood sample and is incubated with the solid phase. They bind to the solid phase in the presence of allergen specific antibodies, when the rest of the serum is washed away. A labeled antibody against the IgE is added, which bounds to any IgE, on the solid phase. The excess is washed away and the levels of IgE are determined.

There are many tips of removing the allergy, but very beneficial tip is to avoid eating those foods which cause allergy, like wheat, peanut or milk. Another tip of reducing the allergy is proper treatment from a doctor or using medications. Especially, if the problem seems to be originating from a food allergy, then testing may reveal that the individual is not allergic to the suspect food, but rather to an ingredient in the food. Peanut oil, for instance, is also a common culprit of allergies. In this modern era, although there are many allergy testing methods used to determine the presence of allergens, but the three most common, famous and beneficial allergy testing procedures are skin tests, elimination tests, and the radioallergosorbent test, which is also called RAST test.

It should be however noted that the accuracy of these test results may vary from each other widely, and even the same test performed at different times may yield different results. But, there is no need to worry, because doctor can understand the pros and cons of varying results and he/she also knows that how it can be controlled.